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ISOLATION AND IDENTIFICATION OF MICROORGANISMS FROM BARBER’S HAIR CLIPPERS
ABSTRACT
This project work was conducted to isolate and identify the microbial contaminants on barbering clipper blades in Umudike and its environs. Twenty Samples were collected from 10 different barbering salons. The samples were collected using a moistened swab sticks and were analysed using standard microbiological procedures. The total bacterial count before and after decontamination of the clipper blades ranged from 6.8×106 to 1.43×107, while the fungal count ranged from 4.5×106-1.2×107. A two-tailed test of the sample means was carried out using the t-distribution to determine the efficacy of the commonly used sterilization technique, which was not significant at P<0.01. Four bacterial and six fungal species were isolated and identified. The four bacteria isolated include; Staphylococcus aureus, Bacillus subtilis, Staphylococcus epidermidis and Streptococcus spp, while Microsporum audouinii, Microsporum canis, Rhizopus spp, Trichophyton spp, Mucor spp and Penicillium spp were the fungal isolates identified. The presence of these pathogens is an indication that barbering practice could be contributing to the spread of infection within Umudike and its environs.
TABLE OF CONTENT:
CHAPTER ONE
INTRODUCTION
1.1 Background of the Study
1.2 Statement of the Research Problem
1.3 Objectives of the Study
1.4 Significance of the Study
1.5 Research Questions
1.6 Research Hypothesis
1.7 Conceptual and Operational Definition
1.8 Assumptions
1.9 Limitations of the Study
CHAPTER TWO
LITERATURE REVIEW
2.1 Sources of Literature
2.2 The Review
2.3 Summary of Literature Review
CHAPTER THREE
RESEARCH METHODOLOGY
3.1 Research Method
3.2 Research Design
3.3 Research Sample
3.4 Measuring Instrument
3.5 Data Collection
3.6 Data Analysis
3.7 Expected Result
CHAPTER FOUR
DATA ANALYSIS AND RESULTS
4.1 Data Analysis
4.2 Results
4.3 Discussion
CHAPTER FIVE
SUMMARY AND RECOMMENDATIONS
5.1 Summary
5.2 Recommendations for Further Study
References
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